The limited host range of an Agrobacterium tumefaciens strain extended by a cytokinin gene from a wide host range T-region

Molecular characterization of a host-range-determining locus from Agrobacterium tumefaciens.

The virulence loci play an essential role in tumor formation by Agrobacterium tumefaciens. This study focused on the virC locus, which affects the host range Agrobacterium species. virC mutants display an attenuated or avirulent phenotype on certain host plants, but remain fully virulent on other plant hosts. The nucleotide sequence revealed that the virC locus of pTiA6NC is an operon consistin...

Agrobacterium tumefaciens increases cytokinin production in plastids by modifying the biosynthetic pathway in the host plant.

Agrobacterium tumefaciens infects plants and induces the formation of tumors called "crown galls" by integrating the transferred-DNA (T-DNA) region of the Ti-plasmid into the plant nuclear genome. Tumors are formed because the T-DNA encodes enzymes that modify the synthesis of two plant growth hormones, auxin and cytokinin (CK). Here, we show that a CK biosynthesis enzyme, Tmr, which is encoded...

A Strain Range Dependent Cyclic Plasticity Model

Hysteresis loop curves are highly important for numerical simulations of materials deformation under cyclic loadings. The models mainly take account of only the tensile half of the stabilized cycle in hysteresis loop for identification of the constants which don’t vary with accumulation of plastic strain and strain range of the hysteresis loop. This approach may be quite erroneous particularly ...

Broadbean wilt virus - identification,host range and distribution in the fars province of iran

Isolation of high-quality RNA from a wide range of woody plants

Isolation of high-quality RNA is one of the most crucial methods in molecular biology. RNA extraction from woody plants has been problematic due to the presence of rigid and woody tissues, large amounts of polysaccharides, polyphenols and other secondary metabolites. Here we present a suitable protocol for RNA isolation from a wide range of woody plants that includes eight gymnosperms and four ...